Noninvasive Fetal Sex Determination Using Cell-Free Fetal DNA
August 10, 2011
Despite interstudy variability, performance was high using maternal blood. Sensitivity and specificity for detection of Y chromosome sequences was greatest using RTQ-PCR after 20 weeks' gestation. Tests using urine and tests performed before 7 weeks' gestation were unreliable.
Risk Free Simultaneous Prenatal Identification of Fetal Rhesus D Status and Sex By Multiplex Real-Time PCR Using Cell Free Fetal DNA in Maternal Plasma
February 10, 2001
By developing a novel multiplex real-time PCR assay we present the first report describing the determination of multiple fetal loci from cell free DNA in maternal plasma by these means. As this assay is suitable for automation, our data, therefore, suggest that such assays provide a good basis for the clinical examination of multiple fetal loci, in particular Rhesus D status or fetal sex, and can be performed effectively using real-time multiplex PCR assays.
Rapid Clearance of Fetal DNA from Maternal Plasma
January 1, 1999
Fetal DNA has been detected in maternal plasma during pregnancy. We investigated the clearance of circulating fetal DNA after delivery, using quantitative PCR analysis of the sex-determining region Y gene as a marker for male fetuses…
Presence of Fetal DNA in Maternal Plasma and Serum
August 16, 1997
Our finding of circulating fetal DNA in maternal plasma may have implications for non-invasive prenatal diagnosis, and for improving our understanding of the fetomaternal relationship.